File Name: ribosomal its sequences and plant phylogenetic inference .zip
We identified eight putative ITS pseudogenes from four taxa with low predicted secondary structure stability and high substitution rates. Maximum likelihood ML and neighbor-joining NJ methods were used for phylogenetic reconstruction. In the former clade, most tetraploid taxa appear to be polyphyletic; sequences from individual plants did not correspond to recognized taxonomic units. This indicates a recent divergence of the East Asian tetraploids. This is a preview of subscription content, access via your institution.
Agarwood is a type of wood that is used in traditional Thai medicines and has been applied in several recipes; in particular, this wood is considered as a costly resinous non-wood product. However, the morphological characteristics of the plant in the genus Aquilaria are quite similar, and so the authentication steps need a botanical expert for the purposes of identification and then also require an efficient tool for species-level discrimination. DNA barcoding is an approved molecular technique which uses a short sequence as a barcoding region specific for identified species. The phylogenetic tree of each locus was reconstructed and the genetic distances were also determined using a maximum likelihood method. Our results showed that all samples in each locus were successfully amplified and sequenced with universal primers. The ITS was only performed with suitable markers for Aquilaria species identification, which were considered from ML phylogenetic tree reconstruction and the optimum length of genetic distance.
Phylogenetic analyses were conducted to evaluate relationships of 7 taxa of Korean Carpesium including three outgroup Inula britannica L. Phylogenetic studies used maximum parsimony, neighbor-joining and maximum likelihood methods analysis. The total number of variable sites was for the entire sequences, and a parsimony informative sites of 64 are valid. As the result, Korean Carpesium were formed monophyletic group and C.
Flowering times in Viburnum. Arnoldia PDF Donoghue, M. Growth patterns in woody plants with examples from the genus Viburnum. PDF Coombs, E.
Separate and combined analyses were performed. ITS-variation was extensive and, because of alignment ambiguities, some sites were excluded from the analyses.
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In this paper, we discuss the possibility of using a short nuclear DNA sequence as a barcode to identify a wide range of medicinal plant species. First, the PCR and sequencing success rates of ITS and ITS2 were evaluated based entirely on materials from dry medicinal product and herbarium voucher specimens, including some samples collected back to 90 years ago. Our results supported the hypothesis that ITS2 can be used as a minibarcode to effectively identify species in a wide variety of specimens and medicinal materials.
Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. DOI: Wendel Published Biology, Medicine Molecular phylogenetics and evolution. One of the most popular sequences for phylogenetic inference at the generic and infrageneric levels in plants is the internal transcribed spacer ITS region of the 18S Expand Abstract.
One of the most popular sequences for phylogenetic inference at the generic and infrageneric levels in plants is the internal transcribed spacer (ITS) region of.
Она посмотрела на него недовольно. В том, что касалось Мидж Милкен, существовали две вещи, которые никому не позволялось ставить под сомнение. Первой из них были предоставляемые ею данные. Бринкерхофф терпеливо ждал, пока она изучала цифры. - Хм-м, - наконец произнесла. - Вчерашняя статистика безукоризненна: вскрыто двести тридцать семь кодов, средняя стоимость - восемьсот семьдесят четыре доллара.
Это на нижнем этаже. Возле фреоновых помп. Сьюзан повернулась и направилась к двери, но на полпути оглянулась. - Коммандер, - сказала. - Это еще не конец.
Правое запястье в гипсе.
Barcode of nuclear ribosomal internal transcribed spacer regions ITS as a useful tool to recognize a newly naturalized and potentially invasive weed, Chloris pilosa Schumach.